Enzymatic degradation of polyhydroxyalkanoate using lipase from Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
A singular enzymatic megacomplex from Bacillus subtilis.
Nonribosomal peptide synthetases (NRPS), polyketide synthases (PKS), and hybrid NRPS/PKS are of particular interest, because they produce numerous therapeutic agents, have great potential for engineering novel compounds, and are the largest enzymes known. The predicted masses of known enzymatic assembly lines can reach almost 5 megadaltons, dwarfing even the ribosome (approximately 2.6 megadalt...
متن کاملMolecular Detection of Lipase A gene in Putative Bacillus subtilis Strains Isolated from Soil
The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A ge...
متن کاملOverexpression and characterization of a lipase from Bacillus subtilis.
A novel plasmid, pBSR2, was constructed by incorporating a strong lipase promoter and a terminator into the original pBD64. A mature lipase gene from Bacillus subtilis strain IFFI10210, an existing strain for lipase expression, was cloned into the plasmid pBSR2 and transformed into B. subtilis A.S.1.1655. Thus, an overexpression strain, BSL2, was obtained. The yield of lipase is about 8.6 mg pr...
متن کاملEnzymatic synthesis of glycosylated puerarin using maltogenic amylase from Bacillus stearothermophilus expressed in Bacillus subtilis.
BACKGROUND The maltogenic amylase from Bacillus stearothermophilus (BSMA) is a valuable biocatalyst that has been used to transglycosylate natural glycosides to improve solubility. To ensure safety, BSMA was produced in Bacillus subtilis, using new shuttle vector-based expression vectors. The transglycosylation of puerarin was also conducted with crude BSMA and analyzed. RESULTS Two expressio...
متن کاملBacillus subtilis A singular enzymatic megacomplex from Kolter
www.pnas.org#otherarticles This article has been cited by other articles: E-mail Alerts . click here at the top right corner of the article or Receive free email alerts when new articles cite this article sign up in the box Rights & Permissions www.pnas.org/misc/rightperm.shtml To reproduce this article in part (figures, tables) or in entirety, see: Reprints www.pnas.org/misc/reprints.shtml To ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Environmental Science and Technology
سال: 2016
ISSN: 1735-1472,1735-2630
DOI: 10.1007/s13762-016-0992-5